Image formation electron dense

Terms used to describe the contrast are electron transparent (lucent) and electron dense. Electron transparent-areas scatter fewer electrons and appear bright in the image, whereas electron dense areas scatter more electrons and appear dark. Discrete areas of electron transparency and electron density, then, are responsible for the contrast required for image formation. 

The structure (e.g., number, size, distribution) of fat crystals is difficult to analyze by common microscopy techniques (i.e., electron, polarized light), due to their dense and interconnected microstructure. Images of the internal structures of lipid-based foods can only be obtained by special manipulation of the sample. However, formation of thin sections (polarized light microscopy) or fractured planes (electron microscopy) still typically does not provide adequate resolution of the crystalline phase. Confocal laserscanning microscopy (CLSM), which is based on the detection of fluorescence produced by a dye system when a sample is illuminated with a krypton/argon mixed-gas laser, overcomes these problems. Bulk specimens can be used with CLSM to obtain high-resolution images of lipid crystalline structure in intricate detail. 

The relation between conductivity, chemical and phase compositions, and the structure of separate phases of solid electrolytes is very important In many systems, the maximum in conductivity closely coincides with the existing F-phase (fluorite-phase) limit TEM image of Figure 13.3 shows the formation of a dense single-phase polycrystalline microstructure for the sintered ceramics [1]. X-ray diffraction and analysis of electron diffractograms of this sintered ceramic confirmed the fluorite structure. 

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