Image analysis staining protocols

This is an entirely gel-based procedure no mass spectrometry is involved. Proteins from the two comparison samples are separated by a 2-DE protocol and images of the stained spots are captured after staining the gels with a suitable dye (e.g., CCB or SYPRO Ruby) next, the measured densities of the spots are compared using image analysis software to provide differential expression of proteins.44 45 Identification of proteins is accomplished as mentioned in Section 9.12.3. 

The combination of a multiple staining protocol with CSLM enabled the visualization of the 3D structure ofthe main components of a biofouling layer (details are reviewed in Section 4.3.3). By applying a complex image analysis, several morphological parameters were determined, such as the porosity and fractal dimension of the fouling layer. 

---