Image acquisition and analysis

Automated Image Acquisition and Analysis in Multiple Staining... 

Gel imager (Gel Doc XR, Bio-Rad) and image acquisition and analysis software (Quantity One 1-D Analysis Software, Bio-Rad). 

Image Acquisition and Analysis Introduction and General Notes... 

Fig. 1. Difference gel electrophoresis (DICE) workflow. An aliquot of each sample is mixed to create the internal standard. All samples and the standard are labeled, and then combined before running the 2-D gel. Image acquisition and analysis complete the workflow. Obtained with permission from Westermeier R, Scheibe B. Difference gel electrophoresis based on lys/cys tagging. In Posch A. Ed. Sample Preparation for 2D PAGE. Methods in Molecular Biology, Humana Press, Totowa, NJ (2007).

Herr, S., Bastian, T., Pepperkok, R., Boulin, C., and Ansorge, W. (1993). A fully automated image acquisition and analysis system for low light level fluorescence microscopy. Methods Mol. Cell Biol. 4, 164-170. 

The experimental set-up for analysis of a sessile-drop system by ADSA-D is similar to that for ADSA-P (see Figure 14.7), except that the imaging system is aligned vertically rather than horizontally. As with ADSA-P, digital image acquisition and analysis is used. The procedures for determining the desired diameter can be performed either manually or automatically (44). 

Pyrz WD, Buttrey DJ. Particle size determination using TEM a discussion of image acquisition and analysis for the novice microscopist. Langmuir. 2008 24 11350-60. 

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