Idiotypic determinants localization

Idiotypic specificities on free L chains have been localized to the variable half of the polypeptide chain by Tan and Epstein, who isolated such fragments from the urine of a patient with multiple myeloma (92). The corresponding, intact Bence Jones protein was also purified and used to prepare antibodies in rabbits which, after appropriate absorption, reacted with idiotypic determinants of the immunogen. A line of identity was obtained in the reaction of these anti-D antibodies with the Bence Jones protein and the fragment comprising the variable region. Thus, all the individually specific determinants appeared to be represented in the variable segment. 

B. Localization of a M or Idiotypic Determinant to the Region of the Combining Site of an Antibody Molecule... 

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