Identification of events and data analysis

Individual binding events between myosin and actin can be identified by monitoring the variance of the data. Myosin binding increases the system stiffness, as it introduces an additional route to the mechanical ground (the microscope covershp). This means that the Brownian motion is reduced. A typical procedure for detecting myosin binding to actin involves  

The data can be analysed to determine, amongst other things, the average size of the myosin powerstroke and the kinetics of the unbinding process. It is also possible to inspect individual binding events more clearly to see if they have consistent features such as a two-phase powerstroke [Box 2 (c)]. 

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