Identification by Chemical Modification of Peptides

The expansion of protein sequence databases, e.g. TrEMBL, SWISS-PROT, NCBInr brought about by genome sequencing projects, dcCTeases the probability of obtaining an imequivocal protein idenffication by PME alone [118], More information like amino add sequence or amino acid composition increases the confidence in any protein identification. Lahm s group [118] defined three ways to reduce this problem  

Acquisition of an other MALDI-MS spectra with optimised parameters. 

Use of a different endoproteinase to generate a different PME, Identification of a short sequence tag using MALDI-PSD and/or ESI-MS/MS. 

The time required for such experiments prevents such methods being used for high throughput protein identification [119]. 

Specific modification of one or more amino acids in a given peptide chain could more easily supply the necessary information. Possible chanical reactions must have the following requirements  

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