Hypothesis ideal testing process

Test the hypotheses listed in Step 8. Hypothesis can be tested using a variety of field, mesocosm, or laboratory test methods. In an ideal situation it should be possible to make predictions based upon known concentrations and then sample that field site in order to confirm effect or no-effect. It may be necessary to rework the risk assessment in order to reduce uncertainty, or a stressor-habitat-effect linkage may be incorrect. Testing the risk predictions allows feedback into the assessment process improving future predictions. 

This best model of QSAR becomes a hypothesis that must be tested. Several possible approaches may be taken to the next steps in the process. Whatever is done, it is very important not to impose bias on the choice of the next substituents. Choose a new set to test the hypothesis. The concept of an ideal substituent set must be maintained. Fill in gaps in observed activity and extend the tested area toward higher activity. Remember that you cannot predict the activity outside the boundaries of what you have tested. 

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