Hypochlorite reagent

The quantitative analysis of thiamine hydrochloride (vitamin Bi), using HPTLC on sUica gel plates with two different mobile phases, was elaborated. After TLC separation, vitamin Bi was derivatized by the use of tert-butyl hypochlorite or potassium hexacyanoferrate (ni)-sodium hydroxide as reagents. The tert-butyl hypochlorite reagent formed yeUow-fluorescing derivatives... 

Purpose, The oxidation of a secondary alcohol to a ketone is explored using the hypochlorite reagent. Steam distillation is used to separate the product from the reaction mixture and chromatographic techniques are used to purify and isolate the cyclohexanone product. 

To a standard volume of this solution, add phenol and sodium hypochlorite reagents. An indophenol blue colour, proportional in intensity to the concentration of ammonia present, is produced. This colour is evaluated spectrophotometrically at 625 nm using a spectrophotometer which has been calibrated against a standard ammonia solution. 

Determine the optical density of the indophenol blue colour produced between 15 and 30 minutes after addition of the phenol and sodium hypochlorite reagents. 

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