Hyphenated separation techniques benefits

However, most of the time, due to the complexity of the food samples and the need to minimize the sample treatment, that is, the chemical separation of the interferences from the compounds of interest, more powerful instrumental measurements, such as 2D spectroscopies, for example EEM fluorescence spectra, or hyphenated separation techniques, for example gas chromatogra-phy/MS (GC/MS), HPLC/DAD or HPLC/MS, are used. As mentioned in previous sections, obtaining a data table per sample is a much more natural scenario for the application of MCR techniques. In these instances, the typical strategy is to perform the simultaneous analysis of data tables related to standards of known concentration together with tables from unknown samples, in which the concentration(s) of the analyte(s) are determined. Either using multiset analysis or multiway analysis, these determinations benefit from the so-called second-order advantage, which means that analytes can be determined in the presence of interferences, even if those are absent from the calibration samples [48]. The reason why this second-order advantage exists is that MCR techniques describe the information of the compounds in separate concentration profiles and spectra, that is, they make a mathematicar separation of the information related to aU compounds in the analysed sample, analytes and interferences. Afterwards, only the information of the profiles related to the analytes is used for quantitation purposes. 

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