Hypervariable domains

Like RhoA, RhoB is also phosphorylated within its hypervariable domain [86]. In 2008, Pradines and colleagues showed that CKl is capable of phos-phorylating RhoB in vitro and in cellulo at serine 185 [86]. This phosphorylation was independent of the nucleotide bound to RhoB because both constitutively active (G14V) and wild-type RhoB were phosphorylated to similar extents [86]. It is currently unknown how this phosphorylation relates... 

CXX motif) [96], This palmitoylation step is necessary for membrane localization of Wrch-1, thereby serving a function similar to prenylation of other small GTPases. Since Wrch-1 is not prenylated, it does not interact with RhoGDI. However, we have shown that phosphorylation of Wrch-1 within its HVD near the lipidated cysteine does drive changes in both protein localization and function [97,98]. This indicates that hypervariable domain phosphorylation has both prenylation-dependent and -independent effects on Rho family activity. 

The initial discovery and description of the constant, variable, and hypervariable domains and elucidation of the primary structures of the immunoglobulins was made possible because of the disease multiple myeloma. In multiple myeloma, a single B-cell clone (mono-clone) synthesizes large quantities of structurally identical or monoclonal antibody molecules. Isolation of immunoglobulin from multiple myeloma patients who were producing structurally different immunoglobulins... 

Assuming that the third lysin intron is at the position shown by the question mark (Figure 15), the exons of lysin correspond to domains revealed by the crystal structure of the monomer (Figure 16). Exon 1 encodes the N-terminal hypervariable domain of... 

Figure 15.12 Schematic diagram of the barrel arrangement of four p strands from each of the variable domains in Fab. The six hypervariable regions, CDR1-CDR3 from the light chain (L1-L3) and from the heavy chain (H1-H3), are at one end of this barrel. (From J. Novotny et al., /. Biol. Chem. 2S8 14433-14437, 1983.)...

T-cell receptors have variable and constant immunoglobulin domains and hypervariable regions... 

The constant domain has a stable framework structure composed of two antiparallel sheets comprising seven p strands, four in one sheet and three in the other. The variable domains have a similar framework structure but comprising nine p strands, five in one sheet and four in the other. The three hypervariable regions are in loops at one end of the variable domain. The variable domains from the heavy and light chains associate through their five-stranded p sheets to form a barrel with the hypervariable loop regions from both domains close together at the top of the barrel. 

The hypervariable regions are clustered in loop regions at one end of the variable domain 30S... 

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