Hypersil Gold

Figure 10.4 Typical UHPLC trace of isoflavones from a soy protein extract monitored at 260 nm using a Hypersil Gold C18 column (100 x 2.1 mm 1.9 pm Waters, Milford, MA) and a 0.5-mL/min flow rate (9600 psi) of a linear gradient of 0.1 % aq. formic acid versus methanol/acetonitrile(l l). FL = flavone (internal standard), other details in figure and in text. Concentrations of ISOFLAVONE peaks (pM) D = 17.66, GLY = 6.27, G = 26.49, D-Mal = 16.76, GLY-Mal = 3.62, D-Ac = 5.87, G-Mal = 26.70, DE = 3.37, GLYCITEIN = 1.74, G-Ac = 8.33, GE = 4.36.

Column Hypersil GOLD Ci8 column (2.1 mm x 150 mm) Detector LC-MS/MS operated in turbo ion spray ionization MRM monitoring ... 

Remane et al., Imipramine, desipramine, Citalopram-d6, Plasma (0.5 mL) LLE butyl acetate/ TF Hypersil GOLD 10 mM ammoniumAPCI+/QqQ... 

Figure 2.22 Speed-up of a gradient separation with transfer from the fully porous stationary phase Hypersil Gold (Thermo Scientific, Runcorn, UK) to the solid core material Accucore RP-MS (Thermo Scientific, Runcorn, UK). Gradient from 65/35 to...

SunFire Zorbax Extend Hypersil GOLD Platinum EPS RP-WeX Ascentis Phenyl... 

Local anaesthetics Hypersil Gold (100 X 2.1 mm) Methanol and water (1 1) 0.1 % HCOOH MBPS 2nM [22]... 

The mass spectrometer was operated via APCI at 21V collision energy, 50 psi ion gas pressure, and 320°C ionization temperature. Under gradient elution of methanol and water with both including 0.1% formic acid at a flow rate 0.25-0.5 mL/ min with 5 pL injection volume, and using Hypersil GOLD and GOLD aQ columns (100 X 2.1 mm i.d., 1.9 pm particle size), aU the previtamins of vitamin D3, Dj, and isotope-labeled vitamin D3 were baseline separated from their corresponding vitanuns. 

Hypersil Gold Silica Thermo Fisher Scientific 1.9, 3, 5 175 220... 

Glycin tomentella Hayata (leaves and roots) 3 flavones, 7 flavonols, 6 flavanones SLE (reflux, 95 % aq. EtOH) Thermo Hypersil GOLD C18 column (250 X 4.6 mm, 5 pm). Gradient 9 % acetic acid (aq.) and MeOH DAD [115]... 

Analytical column 50 mmx3 mmx5 pm Hypersil Gold (Thermo Scientific). 

Speetra System with UV- visible photometric detector in a gradient mode. The chromatography column Hypersil Gold ODS, 150x4mm Thermo Electron Corp., particles size 5 pm ... 

Figure 5.1 h-v plots of 2.6 [xm shell type (Kinetex), 2.7 jjum shell type (Ascentis Express), sub-2 p,m totally porous particles (Acquity BEH and Hypersil Gold), and a monolith column (ChromoUth Fast Gradient). Experiments were conducted on 5-cm-long narrow bore columns in 48/52 ACN/H20 at 35°C, DM = 1.15 X 10-5 cm /s. From Olah, E., et al. J. Chromatogr. A. 2010, with permission. 

Figure 5.10 Impedance time plots of a steroid separation obtained with columns packed with fully porous sub-2 p,m particles (Grace Vision HT C18, Waters Acquity BEH C18, and Hypersil Gold C18) and with a column packed with 2.7 p,m shell particles (Halo C18). From Fekete, S., et al.

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