2-Hydroxypropyl p-nitrophenyl

In spite of the above mentioned Co(EII) compounds, kinetically labile metal complexes may provide fast product/substrate exchange and some of these systems show real catalytic activity. In native dinuclear phosphatases Mg(II), Mn(II), Fe(II/III), or Zn(II) ions are present in the active centers. Although the aqua complexes of the weakest Lewis acids, Mg(H) and Mn(II), show measurable acceleration of e.g. the transesterification of 2-hydroxypropyl p-nitrophenyl phosphate HPNP, [Mn(II)] = 0.004 M, kobs/ uncat = 73 at pH 7 and 310 K, [38] or the hydrolysis of S -uridyluridine (UpU) [39], only a few structural [40] but no functional phosphatase-mimicking dinuclear complexes have been reported with these metal ions. 

Many artificial systems have been designed recently to imitate the function and behaviour of native enzymes - biomimetic chemistry [27]. Among them, calixarene-based receptors bearing one, two or three Zn(II) complexes on the upper rim were prepared as a model for phosphoesterases [28-31]. Dinuclear receptor 25 was reported to enhance the rate of transesterification of the RNA model substrate 2-hydroxypropyl-p-nitrophenyl phosphate more than 20,000 times compared with the non-catalysed reaction. The complexation mode for the phosphate anion can be described as cascade complexation where the anion is coordinated within the cavity formed by two zinc cations. 

The kinetics and cleavage products of 2-hydroxypropyl p-nitrophenyl phosphate (71) have been investigated in methanol containing a di-Zn(II) complex of bis-l,3-A i,A i -(l,5,9 triazacyclododecyl)propane (72) (Scheme 14). " Time-dependent H-NMR spectra of the reaction mixture at... 

The bimetallic complex 2—Zu2 is a highly efficient turnover catalyst of the transesterification of the RNA model compound 2-hydroxypropyl p-nitrophenyl phosphate (HPNP) (Eq. 26.1). A 23,000-fold rate enhancement is observed in the presence of 2—Zn2 (0.48 mM catalyst concentration, 50 % MeCN-20 mM aqueous buffer, pH 7,25 °C) [16]. The two metal ions in 2—Zn2 efficiently cooperate both in... 

Another outstanding study on the usage of an effective calixarene derivative as a biomimetic catalyst for the cleavage of the RNA model compound, 2-hydroxypropyl p-nitrophenyl phosphate was reported by Baldini et al. [35]. They prepared four calix[4]arene derivatives substituted with two to four guadinium moieties at the upper rim of calixarenes, and investigated their catalytic affinities in the cleavage of the RNA model compound 2-hydroxypropyl p-nitrophenyl phosphate in water (see Figs. 27.17 and 27.18). They found that the... 

Fig. 27.18 Cleavage of the RNA model compound 2-hydroxypropyl p-nitrophenyl phosphate in the presence of calixarene-based pseudo-biocatalyst [35]...

Tee s group has reported on the catalysis of enolization of indan-2-one (200) by a-CD, ) -CD, y-CD, hydroxyethyl-) -CD, and hydroxypropyl-) -CD, all of which accelerate the reaction by up to 22-fold but dimethyl-) -CD slows it by about half These workers have also looked at the effect of alcohols on the basic cleavage of m-nitrophenyl hexanoate by )8-CD. Finally, they have been examining the reaction of a-amino acid anions with p-nitrophenyl acetate and hexanoate in the presence of )S-CD. ... 

The optical rotatory dispersion (ORD) curves of the diastereomers of l-(p-nitrophenyl)-3-methylperhydropyrido[l,2-c][l,3]oxazines, obtained from (-)-2-(2-hydroxypropyl)piperidine with p-nitrobenzaldehyde, are nearly mirror images of each other (68CJC1105). The absolute configurations in cyclohexane of the dextrorotatory cw-3,4fl-H-3-phenyl- and trans-3,4fl-H-3-phenylhydropyrido[l, 2-c] [1,3]oxazin-6-ones, containing 3S,AaR and 35,4a5 atoms, respectively, were determined on the basis of the presence of positive Cotton effects (85T2891). 

Chytry, V., Vrana, A. and Kopecek, J., 1977. Synthesis and activity of a polymer which contains insulin covalently bound on a copolymer of iV-(2-hydroxypropyl) methacrylamide and A-methacryloyldiglycyl p-nitrophenyl ester. Macromolecular Chemistry and Physics, 179(2), 329-336. 

A DFT study of the cleavage of a DNA model, p-nitrophenyl methyl phosphate (47), and two RNA models, p-nitrophenyl 2-hydroxypropyl phosphate (48) and phenyl 2-hydroxypropyl phosphate (48 H for NO2), promoted by the dinuclear Zn(II) complex of l,3-bis(l,5,9-triazacyclododec-l-yl)propane formulated with a bridging methoxide (49), was undertaken to determine possible mechanisms for the tfansesterification processes that are consistent with experimental data. The initial substrate-bound state of... 

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