Hydroxylapatite chromatograph

Lectins (proteins and/or glycoproteins of non-immune origin that agglutinate cells, from seeds of Robinia pseudoacacia), M 100,000. Purified by pptn with ammonium sulfate and dialysis then chromatographed on DE-52 DEAE-cellulose anion-exchanger, hydroxylapatite and Sephacryl S-200. [Wantyghem et al. Biochem J 237 483 1986.]... 

Glueckauf, E. and Patterson, E. 1974. Adsorption of some proteins on hydroxylapatite and other adsorbents used for chromatographic separation. Biochim. Biophys. Acta 351, 57-76. 

Numerous purification procedures have been described (for a review on early procedures see78,149). Most authors use chromatographical methods. The most efficient procedures include adsorption chromatography on brushite as the first step as described in detail by Rice et al.141). Because adsorption of phytochrome on brushite columns sometimes fails63,144) other adsorbents like hydroxylapatite were used... 

Figure 10, Preparation of poliovirus protease Infected cell homogenates were chromatographed on PEAF-cellulose, then on hydroxylapatite, and analyzed on SPS-polyacrylamide gels. Upper panel Left side indicates protein content of protease fraction after UEAE cellulose right side shows removal of host proteins by hydroxylapatite (Coomassie Blue staining). Middle...

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