Hydrophobic interaction chromatograph matrix material

Adsorption on finely divided inert materials. These materials offer a large surface area for interaction with protein. Adsorption of protein on charcoal, a nonpolar substance, presumably occurs by hydrophobic interaction and can be used to separate proteins from a fluid matrix. Adsorption on polar substances such as silica, alumina, or hydroxyapatite depends on ionic interactions or hydrogen bonding these substances can be used with buffer elution in chromatographic separations. 

Chromatographic separation relies on the affinity of binding between different components of the API in liquid and the solid matrix column. The API is separated from the impurities by percolating the liquid through chromatographic columns filled with solid phase matrices. The matrices are made of different materials and separate the components on the basis of physicochemical properties such as charge, size and shape, hydrophobic and hydrophilic characteristics, complex formation with certain ions or metals, and interaction with dyes. 

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