Hydrophobic Effect-Based Recognition

Piletsky, S.A. Andersson, H.S. Nicholls, LA. The rational use of hydrophobic effect-based recognition in molecularly imprinted polymers. J. Mol. Recogn. 1998, 11, 94-97. Hughes, M.P. Smith, B.D. Enhanced carboxylate binding using urea and amide-based receptors with internal Lewis acid coordination a cooperative polarization effect. J. Org. Chem. 1997, 62, 4492-4499. 

Methylcytosine The 5-methyl hydrophobic effect was used to inhibit the recognition of double-hehcal DNA by prokaryotic modifying enzymes and a eukaryotic transcription factor via ohgonucleotide-directed triple-helix formation in which ODNs containing m C conferred substantially more efficient inhibition than those with C [22]. Triple-heUx formation also selectively protects the DNA duplex from restriction methylation, and the specificity of 15 base pairs is useful for chromosome mapping. 

The separation factor relies on the design of the active sites on the resins and the solution chemistry. Mechanisms contributing to chemical selectivity reportedly include the hard-soft acid-base principle (HSAB), hydrophilicity/hydrophobicity selectivity, and pore size recognition. These mechanisms have common characteristics and their effects are thus not easy to distinguish. 

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