Hydrolases glycosyl fluorides

In this case the predominance of glycosylation of C4-carbinols over substrate hydrolysis is in a mixture where the molar concentration of water was more than 3000 times that of the a-maltosyl fluoride. In other experiments the substrate concentration could be further reduced at least 5-fold without altering the fact of a substantially greater extent of maltosidic linkage synthesis than hydrolysis. We now are referring to a very dilute 4 mM, solution of substrate with which, if the hydrolase concept were valid, hydrolysis ought to have been predominant. 

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