Hydrogen sensor proteins

Hydrogen Sensor Proteins and the Control of Hydrogenase Expression... 

Hydrogen sensor proteins, 47 289 Hydrogensulfato compounds, sulfuric acid and, 1 405-107... 

Marjory Stephenson and Leonard Stickland named the enzyme in 1931 (Stephenson and Stickland, 1931). There are five types of hydrogenase. Three of these are Ni proteins and one contains iron as the only transition metal. These proteins catalyze the reversible oxidation of dihydrogen gas (Eq. 7). A fifth class of hydrogenase lacks metals and catalyzes Eq. 8, but not Eq. 7. The Ni enzymes are classed into the NiFe, the NiFeSe, and the hydrogen sensors. All of these enzymes are bidirectional, although different enzymes exhibit a catalytic bias toward making or oxidizing H2. 

Enzymes can lose their activity because of protein denaturation and loss of active site.26 Glucose oxidase can also lose its activity when exposed to excessive amounts of hydrogen peroxide. Most enzymes utilized in the fabrication of biosensors for implantation are derived from nonmammalian proteins, which can lead to an allergic response if the enzymes leach (or leak out of the sensor) into the body. Enzyme leaching can result when the attachment or entrapment of the enzyme is not robust. For example, if a method of entrapment via cross-linking polymers is utilized and the reaction does not go to completion during entrapment, then the enzyme can leach out and cause a loss of signal.27... 

---