Hydrogen production inoculum

Kawagoshi, Y., Hino, N., Fujimoto, A., Nakao, M., Fujita, Y., Sugimura, S., and Furu-kawa, K. 2005. Effect of inoculum conditioning on hydrogen fermentation and pH effect on bacterial community relevant to hydrogen production./. Biosci. Bioeng., 100, 524-530. 

Evaluated the effect of environmental factors on hydrogen production using mixed culture. Successfully enriched naturally available mixed seed to culture hydrogen producing bacteria. Enhanced the hydrogen production through preheat treatment of seed inoculum at 70-90°C for 15 - 20 minutes followed by repeated heat treatment of settled returned sludge. 

More dilute hydrogen peroxide (3% vol/vol) can be purchased from pharmacies or supermarkets. Prior to inoculation, one should verify that the sulfite has been fully oxidized by running Ripper titration or other convenient analyses of SO2, as described by Zoecklein et al. (1995). Considering the potential for overoxidation, this technique might best be used where the volume of inoculum is intended to be low. Winemakers should be cautioned that this procedure is not legal for production-scale application. 

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