Hydrogen-deuterium exchange mass spectroscopy

Hydrogen-deuterium exchange mass spectroscopy (HXMS) 44... 

Kamel AM, Zandi KS, Massefski WW. 2003. Identification of the degradation product of ezlopitant, a non-peptidic substance P antagonist receptor, by hydrogen deuterium exchange, electrospray ionization tandem mass spectrometry (ESI/MS/MM) and nuclear magnetic resonance (NMR) spectroscopy. J Pharm Biomed Anal 31 1211. 

Recent experimental developments in coupling IR spectroscopy techniques with mass spectrometry, which allow the structural characterization of isolated and microsolvated protonated aromatic molecules in the gas phase have been summarized.64 Hydrogen-deuterium exchange has been observed at Ha in (59) and some closely related substrates when this ligand is complexed to Cu1 in [2Hg]acetone.65 The process is finely controlled by the precise coordination distance required to form agostic interaction between Cu(I) and the C-H bond and is believed to involve the enol form of [2H6]acetone and a reversible Cu(I) to Cu(III) interconversion. 

Hydrogen spillover can be interestingly investigated with the help of isotopic molecules such as D2 and T2. Exchange reactions between the surface and the gas phase, and equilibration reactions involving hydrogen and deuterium, are easily monitored by IR spectroscopy, chromatography, and mass spectroscopy. 

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