Hydrazide-agarose

The insolubilization of NAD and AMP and the uses of these supports have already been described, as has the use of hydrazide-agarose for immobilization. Other insolubilized nucleotide affinity columns have also been described. For example, Olsen [101] isolated galactosyltransferase from whey using a UDP-Sepharose affinity column. GTP coupled to a hydrazide Sepharose derivative was used to isolate D-erythro-dihydroneopterin triphosphate synthetase, the first enzyme for folate biosynthesis in Lactobacillus plantarum [102]. ATP-agarose has been used in the purification of heavy meromysin, elution being effected with ATP [103]. 

Bioprobe Polymer Agarose Protein A, AL (protein G mimic), hydrazide, FMP... 

Despite the disadvantage of smaller pore size than agarose, the bead form polyacrylamides have many of the features considered desirable in an affinity support. Thus Inman and Dintzis described the formation of a wide range of polyacrylamide derivatives [37]. The initial reactions involved the formation of aminoethyl, carboxylic or hydrazide deriva-... 

Neuraminidase has been immobilized to give a water-insoluble derivative with retention of enzymic activity by covalent reaction with agarose cyclic imidocarbonate, a hydrazide derivative of agarose cyclic imidocarbo-nate, and periodate-oxidized controlled-pore glass (CPG)-glycophase. ... 

Affi-Gel Hz (Hydrazide-derivatized, cross-linked agarose)... 

Hydrazide-activated agarose e.g. Affi-Gel Hz (Bio-Rad) or CarboLink (IHerce. both these matrices are also available as complete kits with all necessaiy components and instructions... 

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