Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

HYBRIDIZATION WITH NUCLEIC ACID PROBES steps involved

A typical DNA array fabrication and application process involves three major steps. First, nucleic acids (the capture sequences or probes) are immobilized at discrete positions on surface activated substrates. Secondly, the resulting array is hybridized with a complex mixture of fluorescently labelled nucleic acids (the target), and thirdly subsequent to hybridization, the fluorescent markers are detected using a high-resolution scanning laser that quantifies the interaction. This chapter focuses on the first of these processes and provides the reader with an overview of substrates, surface activation methods and dehvery systems available for nucleic acid immobilization. [Pg.78]

An electrochemical DNA hybridization biosensor basically consists of an electrode modified with a single stranded DNA called probe [109]. Usually the probes are short oligonucleotides (or analogues such as peptide nucleic acids). The first and most critical step in the preparation of an electrochemical DNA biosensor is the immobilization of the probe sequence on the electrode. The second step is the hybrid formation under selected conditions of pH, ionic strength and temperature. The next step involves the detection of the double helix... [Pg.51]


See other pages where HYBRIDIZATION WITH NUCLEIC ACID PROBES steps involved is mentioned: [Pg.10]    [Pg.321]    [Pg.228]    [Pg.549]    [Pg.79]    [Pg.5097]    [Pg.293]    [Pg.196]   
See also in sourсe #XX -- [ Pg.107 ]




SEARCH



Acidic probe

Acidity hybridization

Acidity probe

Hybrid nucleic acids

Hybridization probe

Nucleic Acid Hybridization Probes

Nucleic acid hybridization

Nucleic acid probes

Steps involved

© 2024 chempedia.info