Hybridization, PCR

PCR can be used to introduce labels that can then be used for detection. The ability to add to the 5 end of the primers sequences not complementary to the target template, which then becomes incorporated into the double-stranded PCR product, allows the introduction of labels. Thus, the addition of biotin to the 5 end of the primer allows detection of hybridized PCR product with streptavidin or avidin-enzyme conjugates (B4). Other labels such as digoxigenin can be added to the 5 end of the primer, amplified, and detected either colorimetrically or by chemiluminescence (F3). 

Amagliani, G., Omiccioli, E., del Campo, A., Bruce, I. ]., Brandi, G., and Magnani, M. (2006). Development of a magnetic capture hybridization-PCR assay for Listeria monocytogenes direct detection in milk samples. /. Appl. Microbiol. 100, 375-383. 

T.T. Nikiforov, R.B. Rendle, M.L. Kotewicz, Y.H. Rogers, The Use of Phosphorothioate Primers and Exonuclease Hydrolysis for the Preparation of Single-stranded PCR Products and Their Detection by Solid-phase Hybridization , PCR Meth. Appl., 3(5), 285-291 (1994). 

Livak, K. Flood, R Marmaro, J. Giusti, W. SDeetz, K. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR products and nucleic acid hybridization. PCR Methods Appl. 1995, 4. 

Vector genome Dot-blot hybridization PCR [195] Determine genome-containing vector concentration... 

EBV, Epstein-Barr virus FISEI, fluorescence in situ hybridization EIPV, human papilloma virus ISEI, in situ hybridization PCR, polymerase chain reaction RT, reverse transcriptase. 

Hybridize PCR-amplified DNA to allele-spedfic oligonucleotides (ASO) on dot-blot, slot-blot or... 

Methods BF = bright field LM of tissue impression smears, wet-mounts, stained whole mounts LM = light microscopy PH = phase microscopy DF = dark-field microscopy TEM/SEM = transmission/scanning electron microscopy of sections or of purified or semi-purified virus ELISA = enzyme-linked immunosorbent assay PAb = polyclonal antibodies MAb = monoclonal antibodies DBH = dot blot hybridization ISH = in situ hybridization PCR = polymerase chain reaction RT-PCR = reverse transcription PCR. 

Method abbreviations AB = antibody-based method DBH = dot blot hybridization ISH = in situ hybridization PCR = polymerase chain reaction RT-PCR = reverse transcription PCR. 

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