Hyaluronic acid bovine nasal cartilage

Highly purified proteoglycan fractions Al-Dl-Dl and Al-Dl isolated from bovine nasal cartilage was used in this experiment. The samples had been determined to be free of contamination by hyaluronic acid or link protein. Details of isolation and purification of these fractions are described in the literature. Hyaluronic acid sample from Rooster s Comb was generously supplied by Dr. Swann, Harvard Medical School. An Al-Dl subunit fraction which had been treated with CNBr was supplied by Dr. E. 

Proteoglycans and hyaluronic acid have been solubilized from bovine nasal cartilage using lanthanum chloride solutions. The isolated proteoglycans exhibit chemical and physical properties identical to those of similar products isolated by conventional techniques. 

The protein core from keratan sulphate-containing proteoglycan of bovine nasal cartilage has been purified by affinity chromatography on matrix-bound hyaluronic acid. ... 

Chondroitin sulfate could be separated from the chondromucoid. It remained in solution at pH 7 when chondromucoid was shaken with chloroform - amyl alcohol, whereas a second carbohydrate component (possibly keratosulfate or a closely related substance) remained with the protein fraction (4). Electrophoretic studies of chondromucoid show that the components associate in one kind of complex over the pH range 5 to 9 and that a different type of complex occurs below pH 4.85 (71). Shatton and Schubert (7 ) found both chondromucoid and free chondroitin sulfate in the extracts of bovine nasal cartilage the mucoid was extracted first. Protein denaturants did not separate the protein portion of the mucoid. The protein was not collagen (72). Since hyaluronic acid does not appear to... 

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