Hyaluronic acid acetyl groups

Hyaluronic Acid by Group A Streptococcus. III. Origin of iV-Acetyl-glucosamine Moiety. J. biol. Chemistry 212, 583 (1955)-... 

Hyaluronic acid is a glycos uninoglycan polysaccharide composed of D-glucuronic acid (G), which possesses a carboxylate anion, and N-acetyl-glucosamine (N), which possesses an amide group, with linkages 8(1 3) for G-N and 8(1 -> 4) for N-G. In... 

Vibrational frequencies for sulphate and acetyl groups and glycosidic linkages in chondroitin 4- and 6-sulphates and hyaluronic acid, have been identified from Raman spectra. Differences between the spectra for the polysaccharides were interpreted in terms of either the geometry of various substituents, or differences in chemical composition. 

Treatment of hyaluronic acid with hydrazine removed some of the A -acetyl groups, which were reconstituted on reacetylation with tritiated acetic anhydride the tritiated mucopolysaccharide was used as a substrate in a semimicro assay for hyaluronidase. Tritium-labelled hyaluronic acid, labelled by the tritium-recoil technique, has been treated with j8-D-glucuronidase to yield tritiated oligosaccharides containing terminal, non-reducing 2-acetamido-2-deoxy-j3-D-glucopyranosyl residues. ... 

For the polysaccharide synthesis, enzymatic polymerization has been developed as a new in vitro synthesis method of natural and unnatural polysaccharides having complicated structures.The method utilizes a hydrolysis enzyme to catalyze the bond formation for the polymer construction, a reverse direction of the hydrolysis to cleave the bond. This catalysis is due to the enzymatic characteristics, where enzymes catalyze the reverse reaction involving a common intermediate in both forward and backward reactions. In nature, there are many polysaccharides having N-acetyl groups called mucopolysaccharides such as chitin, hyaluronic acid (HA), and chondroitin (Ch). 

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