HUVEC cells

As of today, Cediranib (AZD2171) has the potential to be a best in class angiogenesis therapy. It is one of a new generation series of orally available, highly potent inhibitors of KDR kinase. The compound inhibited VEGF-stimulated HUVEC cell proliferation with an IC50 value of 0.4 nM and was specific for this type of proliferation. 

Shi et al. reported on the synthesis and antiangiogenic activities of lino-mide and its analogs (compounds 11-16) [32], Three of the analogs are 3.3-69 times more potent than linomide at inhibiting blood vessel formation in the chicken chorioallantoic membrane (CAM) [41,42] angiogenesis assay. These compounds possessed considerable antiproliferative activities against isolated human umbilical vein endothelial (HUVEC) cells along with with no... 

VEGF-ERK1/2 phosphorylation in human HUVEC cells 60... 

Kanayama N, Maehara K, Suzuki M, Fujise Y, Terao T. The role of chondroitin sulfate chains of urinary trypsin inhibitor in inhibition of LPS-induced increase of cytosolic free Ca2+ in HL60 cells and HUVEC cells. Biochem Biophys Res Commun 1997 238 560-564. 

In general, any adherent cell type may be used following these same protocols. COS-7 and HUVEC cell protocols are shown as examples of an easier-to-transfect mammalian cell line and more difficult-to-transfect human primary cells, respectively. Typical transfection of COS-7 cells using polymer C32 is 90-100% whereas for HUVECs it is 40-50%. 

The physicochemical differences between the various types of ARSL which were prepared and studied, were found to influence their in vivo kinetics (14) and their antiparasitic/antitrypanocidal activity (15, 16), but not their in vitro anticancer activity, at least not to a substantial level (17) (Table 2). In a series of in vitro studies (17-19), on several types of cancer cell lines (HL-60, C6, PCS, NB4, CHS), it has been demonstrated that all the types of ARSL studies (Table 2) have increased cytotoxicity towards cancer cells (as measured after 24 or 48 h of co-incubation) in comparison with normal cell types (as HUVEC cells) evaluated under the same experimental conditions. 

Fig. 3 (a, b) SEM micrographs of HUVEC cells on fibronectin-coated staich/PCL scaffolds, showing the nano/microfiber combined scaffold after 3 days of culture. Note the ability of the ECs to use the nanofibers to span across the microfiber structure (Adapted from [54])... 

An effect of Vinca alkaloids that may also be important in their anti tumour activity is their antivascular action. It has been shown that vincristine and vindesine are able to reduce the capillaiy network formation by HUVEC cells cultured on Matrigel at non-cytotoxic concentrations, while vinblastine and vinorelbine produce anti-angiogenic effects by direct cytotoxicity [185]. Vinflunine seems to have an antivascular activity consistently superior to that of vinorelbine [25]. 

Antiadhesion compounds are expected to be effective in the treatment of metastasis and arthritis. Macrosphelide C (148) was previously reported to be an HL-60 and HUVEC cell adhesion inhibitor, with ICso values of... 

FIGURE 5.3.10 Density of adherent HUVEC cells on hydrogels. P < 0.01 versus control/IL-1 a (+) P < 0.01 versus ManM(-)/IL-1a(+) P < 0.01 versus ManM(+)/IL-1a(-). Reprinted from Ref. [50], with permission from Copyright 2013... 

EGCG inhibits angiogenesis by enhancing FOXO transcriptional activity. Inhibition of AKT and MEK kinases synergisticaUy induced FOXO transcriptional activity, which was further enhanced in the presence of EGCG. Phosphorylation-deficient mutants of FOXO induced FOXO transcriptional activity and inhibited HUVEC cell migration and capillary tube formation. Inhibition of FOXO... 

In another study, neuroblastoma, human cortical neuronal cells, 3T3, and human umbilical vein endothelial (HUVEC) cells were cultured onto two types of mesoporous silicon with average pore... 

---