Humicola lanuginosa lipase

Humicola lanuginosa lipase (ITIB) [50] Serl46 Leul47 Ser83 Ser83 OG ... 

The Accurel EPIOO carrier, macro porous polypropylene granulates, was obtained from Akzo Nobel. Particle size was in the range of 200-1000 m. 1 gram of carrier was washed with 96% ethanol and water prior to the immobilization. Lipase solution (purified and concentrated solution of Humicola lanuginosa lipase (103 KLU/ml), pH 7.5) was added to the wet carrier. The lipase loadings were in the range of 125-500 KLU/g carrier. The amount of adsorbed lipase activity was calculated from the difference in lipase activity in the supernatant before subtracted the lipase activity after adsorption. 

Kreilgaard, L, Frokjaer, S., Flink, J.M., Randolph, T.W., Carpenter, J.F. Effects of additives on the stability of Humicola lanuginosa lipase during freeze-drying and storage in the dried solid. J. Pharm. Sci. 88, 281-290,1999... 

Yapoudjian, S., Ivanova, M.G., Brzozowski, A.M., Patkar, S.A., ViND, J., SvENDSEN, A. and Verger, R. (2002) Binding of Thermomyces (Humicola) lanuginosa lipase to the mixed micelles of cis-parinaric acid/NaTDC. Eur. J. Biochem. 269, 1613-1621... 

Table 11.1-14. Lipase-catalyzed enantiomer-differentiating hydrolysis of esters of racemic primary alcohols in aqueous solution (PPL pig pancreas lipase, PCL Pseudomonas cepacia lipase, PCL-A Pseudomonas cepacia lipase, Sumitomo, PSL Pseudomonas sp. lipase, PAL Pseudomonas aeruginosa lipase, HLL Humicola lanuginosa lipase, CAL-B Candida antarctica B lipase, CRL Candida rugosa lipase).

The interaction between Humicola Lanuginosa lipase HLL) [57] and sodium dodecyl sulphate (SDS) has been investigated measuring neutron reflectivity from deuterated SDS dissolved in a solution of protonated lipase in D2O. The lipase was found to adsorb readily at the air-water interface but SDS can displace lipase from the interface at higher SDS concentrations [164]. Also, the interaction of dissolved HLL with monolayers of MOG and DPG was studied by GIXD and XR [27]. 

Brzozowski AM, Savage H, Verma CS, et al. 2000. Structural origins of the interfacial activation in Thermomyces (Humicola) lanuginosa lipase. Biochemistry 39 15071-15082. 

A lipase-catalyzed kinetic resolution was described by Parve et al. (89). Bicyclo[3.2.0]heptanol esters were hydrolyzed in SCCO2 with the lipase from Humicola lanuginosa at 40°C and 200 bar. From the racemic ester in Eq. (12), a 59% isolated yield of the 1R,2S,3S,5R ester (67% e.e.) and a 3% yield of the 1S,2R,3R,5S alcohol (92% e.e.) were obtained after 100 h ... 

Immobilized lipase from Humicola lanuginosa Entrapment on polyurethane Hydrolysis of olive oil... 

Omar IC, Hayashi M, Nagai S (1987) Purification and some properties of a thermostable lipase from Humicola lanuginosa No. 3. Agric Biol Chem 51(1) 37-A5 Pacheco-OUver M, Veeraragavan K, Braendfi E (1990) Separation of colour compounds from lipase in fermentation supernatant by diafiltration. Biotechnol Tech 4(l) 369-372 Pandey A, Selvakumar P, Soccol CR et al. (1999) Solid state fermentation for the production of industrial enzymes. Curr Sci 77(1) 149-162... 

Crooks, G.E., Rees, G.D., Robinson, B.H., Svensson, M., Stephenson, G.R. 1995. Comparison of hydrolysis and esterification behavior of Humicola lanuginosa and Rhizomucor miehei lipases in AOT-stabilized water-in-oil microemulsions 11. Effect of temperature on reaction kinetics and general considerations of stability and prodnctivity. Biotechnol. Bioeng. 48, 190-196. 

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