Humicola insolens cutinase

Fig. 3 Top row. Hydrolysis of PET with 7% crystallinity with a Humicola insolens cutinase for (a) 0, (b) 12 and (c) 48 h resulting in weight losses of 0, 18% and 54%, respectively. Bottom row. Hydrolysis of PET with (a) 34.8% crystallinity (blank), (b) a lipolytic enzyme (no weight loss) and (c) 1 M NaOH (modified from [14, 18])...

The polymerization of substrates varying in a,(o-n-alkane diol and a,co- -alkane diacid chain length by Humicola insolens cutinase immobilized on Amberzyme oxiranes (HiC-AO) and Novozym 435 was studied [39], HIC-AO... 

Apart from CALB other enzymes were shown to be able to successfully polymerize lactones. For instance Lipase PS-30, immobilized on Celite, was used as catalyst to study PDL-ROP under bulk reaction conditions. Poly(PDL) with M = 62000 and PDI 1.9 was reported [76]. Gross and coworkers could show that Humicola insolens cutinase (HiC) showed a high catalytic activity for enzymatic ROP of e-CL and PDL [87]. Poly(e-CL) with M n = 16000 (M /lVf = 3.1), in >99% yields was produced in bulk (70 °C, 24h) with 0.1% w/w immobilized HiC. Furthermore, using immobilized HiC in toluene (70 °C, 24h), PDL was converted to poly(PDL) (99% yield) with M = 44600 and Mw/M = 1.7. 

Humicola insolens cutinase immobilized on Amberzyme oxiranes... 

Diacids (succinic acid, adipic acid, suberic acid, and sebacic acid), diols (cyclohexane-1,4-dimethanol, 1,8-octanediol, 1,6-hexanediol and 1,4-butane-diol), and lactone (co-pentadecalactone) were purchased from Aldrich Chemical Co. in the highest available purity and used as received m-Caprolactone, a gift from Union Carbide, was dried over calcium hydride and distilled under reduced pressure in a nitrogen atmosphere. Humicola insolens cutinase was a gift from Novozymes (Bagsvaerd, Denmark). Lewatit OC VOC 1600 was received as a gift from Rohm and Haas. 

A. D. Nielsen, L. Arleth, and P. Westh, Analysis of protein-surfactant interactions—a titration calorimetric and fluorescence spectroscopic investigation of interactions between Humicola insolens cutinase and an anionic surfactant, Biochimica et Biophysica Acta, vol. 1752, no. 2, pp. 124—132, 2005. 

As described by Hunsen and co-workers [31], cutinase HiC (cutin hydrolase from Humicola insolens) catalysed a dehydration polycondensation between a glycol and a diacid at 70 °C for 48 h under vacuum, containing 1 % w/w enzyme. With the adipic acid component fixed, polyesters with M values up to 12,000 Da were obtained from diols. With the 1,4-cyclohexanedimethanol component fixed, polyesters obtained from succinic acid, adipic acid, suberic acid and sebacic acid had Mn values of 900, 4,000, 5,000 and 19,000 Da, respectively. 

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