Human serum albumin-drug analysis

Hanai, T., Miyazaki, R., and Kinoshita, T., Quantitative analysis of human serum albumin-drug interactions using reversed phase and ion-exchange liquid chromatography, Anal. Chim. Acta, 378, 77, 1999. 

The frontal analysis technique has been used for the determination of enantioselective binding constants of chiral drugs such as warfarin, verapamil, nilvadipine, and semotidil with proteins such as bovine serum albumin (BSA), human serum albumin (HSA), and plasma lipoproteins (45-51). 

Kraak et al. (38) reported the first ACE application to study drug binding to a plasma protein. They used the model system warfarin-human serum albumin (HSA) to compare the suitability of the Hummel-Dreyer, frontal analysis, and vacancy peak methods. A more methodologically intended paper from Erim and Kraak (39) used VACE to study the displacement of warfarin from bovine serum albumin (BSA) by furosemide and phenylbutazone. They concluded that VACE is especially suited to examining competitive properties of simultaneously administered compounds toward a given protein-drug system. 

Erostell-Karlsson, A., Remaeus, A., Roos, H., Andersson, K, Borg, P., Hiimalainen, M. and Karlsson, R. (2000) Biosensor analysis of the interaction between immobilized human serum albumin and drug compounds for prediction of human serum albumin binding levels. Journal of Medicinal Chemistry, 43, 1986-1992. 

Petitpas I, Bhattacharya AA, Twine S et al. (2001) Crystal structure analysis of warfarin binding to human serum albumin. Anatomy of drug site I. J Biol Chem 276 22804—22809 Sadowski J, Rudolph C, Gasteiger J (1992) The generation of 3D models of host-guest complexes. Anal Chim Acta 265 233-241... 

QSRR analysis of HPLC data determined on an immobilized human serum albumin (HSA) column helped to propose the topography of two binding sites of different affinity to benzodiazepine enantiomers 1143.163). Also, the mechanism of interaction of phenothiazine neuroleptics with melanin was rationalized by means of QSRR analysis of HPLC retention data [132,164]. Another QSRR study concerned the interactions of drugs with immobilized keratin and collagen [ 165). 

Test strips, which are available for the determination of about ten low-molecular mass substances (metabolites, drugs, and electrolytes) and eight enzymes [356], can be considered as precursors of optoelectronic biosensors. Efficient optoelectronic sensors based on immobilized dyes have been devised for the determination of glucose, urea, penicillin, and human serum albumin [357]. Other approaches use immobilized luciferase or horseradish peroxidase to assay ATP or NADH or, when coupled with oxidases, to measure uric acid or cholesterol. These principles have not yet been generally accepted for use in routine analysis. Thermistor devices involving immobilized enzymes or antibodies for a number of clinically relevant substances have also been described. Thermometric enzyme linked immunosorbent assays are being routinely employed for monitoring the production of monoclonal antibodies. 

J. R. Simad, P. A. Zunszain, J. A. Hamilton and S. Curiy, Location of high and low affinity fatty acid binding sites on human serum albumin reveled by NMR drug-competition analysis,/. Mol. Biol, 2006, 361,336-351. 

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