Human gastric lipase

Abbreviations GcL, Geotrichum candidum lipase hPL, human pancreatic lipase RmL, Rhizomucor miehei lipase hHL, human hepatic lipase hLPL, human lipoprotein lipase hLAL, human lysosomal acid lipase hGL, human gastric lipase BAL, bile salt-activated lipase HSL, hormone-sensitive lipase CLP, colipase AChE, Torpedo cal omica acetylcholinesterase cDNA, complementary deoxyribonucleic acid VLDL, very low-density lipoprotein IDL, intermediate-density lipoprotein HDL, high-density lipoprotein apoC-II, apolipoprotein C-II. 

The cDNA-derived amino acid sequences of human gastric lipase (Bodmer et ai, 1987) and rat lingual lipase (Docherty et al., 1985) show clear homology, with 78% identity. There are 379 and 377 amino acids, respectively, and three of the four putative glycosylation sites in the human enzyme were also found in the rat lingual protein. However, with the exception of the G-X-S-X-G consensus sequence motif, there appears to be no homology with any other lipase family. 

R. and Lowe, P.A. (1987) Molecular cloning of a human gastric lipase and expression of the enzyme in yeast. Biochim. Biophys. Acta 909, 237-244... 

Roussel, A., Canaan, S., Egloff, M. P., Riviere, M., Dupuis, L, Verger, R. and Cambillau, C. (1999) Crystal structure of human gastric lipase and model of lysosomal acid lipase, two lipolytic enzymes of medical interest. J. Biol. Chem. 274, 16995-17002... 

Gargouri, Y, Moreau, H., Pieroni, G. and Verger, R. (1988) Human gastric lipase A sulfhydryl enzyme. J. Biol. Chem. 

Miled, N., Roussel, A., Bussetta, C., Berti-Dupuis, L, Riviere, M., Buono, G., Verger, R., Cambillau, C. and Canaan, S. (2003) Inhibition of dog and human gastric lipases by enantiomeric phosphonate inhibitors a structure-activity study. Biochemistry 42, 11587-11593... 

Immunocytolocalisation of human gastric lipase in chief cells of the fundic mucosa. LListochemistry 91, 419-423. 

R. (2001) Effects of Lansoprazole on Human Gastric Lipase Secretion and Intragastric Lipolysis in Healthy Human Volunteers. Digestion 63, 207-213. 

Y. Gargouri, G. Pieroni, C. Rivifere, P. Lowe, J. Saunifere, L. Sarda, and R. Verger. Importance of human gastric lipase for intestinal lipolysis an in vitro study. Biochim. Biophys. Acta 879 419 (1986). 

PPL Therapeutics Bile human gastric Lipase, Fibrinogen, thrombin, Factor VII, Factor IX, alpha-antitrypsin, calcitonin (salmon), collagen, superoxide dismutase, Glucagon lipopeptide, Human serum albumin, Protein C 59-63... 

Smerdon GR, Aves SJ, Walton EE. Production of human gastric lipase in the fission yeast Schizosaccharomyces pombe. Gene, 1995 165(2) 313-318. 

Human milk lipase and human gastric lipase have specificities similar to pancreatic lipase and hydrolyze tributyrin more rapidly than other triglycerides (240, 243). Triglycerides with a chain length of four to eight carbon atoms are hydrolyzed most rapidly by Ricinus lipase (146). Specificity similar to pancreatic lipase is shown by the lipase of Penicil-litim roqueforti (244), but the lipase of Aspergillus hydrolyzes tricaprylin and triolein more rapidly than tributyrin (244). 

M. W. Bodmer, S. Angal. G. T. Yarranton, T. J. R. Harris. A Lyons, D J King, G. Pieroni.e. Riviere, R. Verger, P. A. Lowe. Molecular cloning of a human gastric-lipase and c.xpression of the enzyme in years. Biochim. Biophys Acta 909 2.37-244. 1987. 

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