Human cerebrospinal fluid, reverse-phase

Fig. 11.8.4. HPLC of thiamine in human serum (a) and human cerebrospinal fluid (b). Chromatographic conditions stationary phase, pBondapak C18 (10 pm) reversed phase (300 X 3.9 mm I.D.) mobile phase, methanol-aqueous sodium citrate, pH 4.0, 0.05 mol/1 (45/55, v/v), sodium 1-octanesulphonate 10 mmol/1 temperature, ambient flow rate, 1.2 ml/min detection, post-column fluorescence (excitation at 367 nm, emission at 435 nm). Peaks 1, saUcylamide 2, thiamine. Reproduced from Wielders and Mink (1983), with permission.

An assay has been described for the determination of vandetanib in human plasma and in cerebrospinal fluid. The assay consists in an LLE with tert-butyl methyl ether in the presence of ammonium hydroxide, followed by evaporation of the top organic layer and reconstitution in ACN/10 mM ammonium formate pH 5, prior to reversed-phase LC tandem MS [118],... 

JPM Wielders, CJK Mink. Quantitative analysis of total thiamine in human blood, milk and cerebrospinal fluid by reversed-phase ion-pair high-performance liquid chromatography. J Chromatogr 277 145-156, 1983. 

---