HPLC Methods in Dissolution Testing

Development of efficient and reliable HPLC methods for dissolution testing can at times be problematic. Several factors need to be taken into consideration during method development. 

FIGURE I Typical fast HPLC chromatograms for a set of three dissolution samples at three time points. 

Overall, a broad choice of detection methods in HPLC has provided pharmaceutical researchers with great flexibility in solving the dissolution 

TABLE I Compatibility of Commonly Used Dissolution Media with HPLC Methods 

Dissolution media UV detection Other selective methods (electrochemical, fluorescence, etc.) Mass spectrometric detection 

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