HPAE

Jahnel, J. B., P. Ilieva, and F. H. Frimmel. 1998a. HPAE-PAD — A sensitive method for the determination of carbohydrates. Fresenius Journal of Analytical Chemistry 360 827-829. 

Keywords autonomous wind-hydrogen station (AWHS), wind power station (WPS), high-pressure alkaline electrolyser (HPAE)... 

Jahnel J. B., IhevaP., and Frimmel F. H. (1998) HPAE-PAD— a sensitive method for determination of carbohydrates. Fres. 

Carbohydrate Analysis Following glycoprotein hydrolysis, dilute sample 1 1 with DIW and speed vacuum dry well to remove residual TEA. To acheive the required pH 5, add 100 pi of 20 mM sodium acetate (pH 5) and if necessary, adjust pH between 4 and 5 with NaOH. The hydrolysate containing monosaccharides is transfered to a pre-wetted Microcon-SCX and centrifuged for 30 sec at high speed to remove free amino acids and unhydrolyzed peptides for HPAE-PAE analysis by electrochemical detection. 

HPAE-PAD high pH anion exchange with pulse amperometric detection... 

HPLC with fluorescence or UV detection either on a C18 column using a gradient of acetonitrile / sodium acetate (aq) or on HPAE-FD. 

Alternatives to DMB analysis include HPLC of OPD (o-phenylenediamine) labeled sialic acids [6] as well as HPAE-PAD and hybrid variants of these procedures. 

Oligosaccharide Oligosaccharide Oligosaccharide HPAE Profile Charge Profile CE Profile... 

Analysis of Carbohydrates by HPAE-PAD, Technical Note 20, Dionex, 1993. 

Figure 13.14 Schematic diagram of HPAE-PAD system. ( Thermo Fisher Scientific (www.thermofisher.com). Used with permission.)...

The elucidation of a levan polysaccharide produced by a novel halophilic isolate from soil samples taken from Camalti Saltern area in Turkey, Halomonas smyrniensis was achieved thanks to the glycosyl analysis. In particular, the microbial EPS was hydrolyzed with trifluoroacetic acid (0.5M TFA at temperature of 120°C for 1.5h) and subsequently analyzed by HPAE-PAD (High-Performance Anion-Exchange Chromatography Pulsed Amperometric Detection), a high-performance anion-exchange... 

Figure 16 Structure of MPEG-HPAE block copolymer. DOX-loaded MPEG-HPAE micelles under physiological pH were completely dissociated and rapidly released DOX in weakly acidic pH environments. (Reproduced from Ref. 73b. Elsevier, 2007.)...

Figure 3.230 Schematic of a HPAE-PAD system with vacuum degassed electrolytically generated hydroxide eluents.

HPAE-PAD (high-performance anion-exchange chromatography with pulsed amperometric detection) was successfully employed as a simple method for the analysis of free or bound oligosaccharides (see Section 3.10.3). This method can... 

The following example emphasizes the selectivity of the HPAE-PAD method in a different way. Figure 3.256 shows the separation of the oligosaccharides listed in Table 3.32 that can exist in glycoproteins. 

Although structures 3, 10, and 12 exhibit different compositions, their hydro-dynamic volumes are very similar. The hydrodynamic volume of an oligosaccharide can be determined via the determination of glucose equivalents by means of ion-exclusion chromatography. Structures 10 and 12, which differ by only 0.1 glucose equivalents, can easily be separated by the HPAE-PAD method, whereas the separation by ion-exclusion chromatography is extremely difficult. 

It can also be seen from Figure 3.256 that the HPAE-PAD method can be applied for the separation of linkage isomers of charged oligosaccharides. The... 

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