Horizontal chambers isocratic

Isocratic linear development is the most popular mode of chromatogram development in analytical and preparative planar chromatography. It can be easily performed in horizontal chambers of all types. The mobile phase in the reservoir is brought into contact with the adsorbent layer, and then the movement of the eluent front takes place. Chromatogram development is stopped when the mobile phase front reaches the desired position. Usually 20 X 20 cm and 10 X 20 cm plates are applied for preparative separations, and this makes the migration distance equal to about 18 cm. Due to the fact that the migration distance varies with time according to the equation Z, = (Z, c, and t are the distance of the solvent front traveled, constant,... 

The horizontal DS chamber has been described for use in isocratic, gradient, and multiple development, as well as simultaneous development using different mobile phases under different conditions (Waksmundzka-Hajnos and Wawrzynowicz, 1994 Matysik, 1994 Matysik and Wojtasik, 1994 Matysik et al., 1994 Golkiewicz, 1996 Matysik and Toczolowski, 1997). A modified version of the DS chamber allows the solvent entry position to be changed by movement of the plate, increasing efficiency in single and multiple development (Dzido et al., 1995). 

---