Homogeneity Distribution in Size and Shape

Homogeneity Distribution in Size and Shape. In 1950, Shabp and Beabd demonstrated that Stokes law could be used to determine the size of spherical latex particles of known density. Particles were sedimented in solvents with various densities and their size calculated from an equation relating the radius of the 

Equation (1) may then be expressed as a relationship between 8, r, and Aq, the difference between particle and solvent densities. 

Good agreement was obtained between sedimentation velocity and other sizing measurements for particles 253 m in diameter. This diameter is in the same size range as the chylomicron particle. 

Dole and Hamlin (1962) used Stokes law to describe the lower limit of particle size for chylomicron fractions separated by ultracentrifugal dotation under carefully controlled conditions. They related the distance of flotation (%) fhe particle diameter (d) to A q 7 and 0) x Gniin. 

Two nomograms were prepared. The first calculated xjd as a function of AqJt] and Gmin. The second calculated d as a function of X- These nomograms are extremely important since they facilitate particle size calculations for the many different chylomicron fractions which have been described in the literature (Cornwell and Kruger 1961a, Dole and Hamlin 1962). Dole and Hamlin correctly emphasize that chylomicron preparations should be described by the Gmin. used and the particle size limit obtained. 

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