Histochemical marker genes

Here follow protocols to detect histochemical marker genes in cells or tissues. 

Lin, W. C., Pretlow, T. P., Pretlow, T. G. and Culp, L. A. (1992). High resolution analyses of two different classes of tumor cells in situ tagged with alternative histochemical marker genes. Am. J. Pathol. J4J, 1331-1342. 

Culp, L. A., Lin, W., Kleinman, N. R., O Connor, K. L. and Lechner, R. (1998). Earliest steps in primary tumor formation and micrometastasis resolved with histochemical markers of gene-tagged tumor cells. J. Histochem. Cytochem. 46, 557-568. 

Using a vector carrying a splice acceptor sequence placed upstream of a reporter gene (such as lacZ or GFP [green fluorescent protein]), different types of regulatory or gene sequences can be trapped (31-33). ES cell-chimeric embryos are stained for the histochemical marker to reveal expression domains of the tr jped elements (31-33). On the basis of the expression pattern information... 

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