Hippocampal slice preparation

In order to circumvent the difficulties of maintaining known, constant dmg concentrations in the brain in vivo, the hippocampal slice preparation was adapted to study the acute anatomic effects of psychotropic dmgs. This method was implemented in conjunction with Drs. K. Stratton and... 

Kemp, J. A., Marshall, G. R., and Woodruff, G. N. (1986) Quantitative evaluation of the potencies of GABA-receptor agonists and antagonists using the rat hippocampal slice preparation. Br. J. Pharmacol. 87,677-684. 

Rebola R, Oliveira CR, Cunha RA (2002) Transducing system operated by adenosine A2A receptors to facilitate acetylcholine release in the rat hippocampus. Eur J Pharmacol 454 31-8 Reddington M, Lee KS, Schubert P (1982) An Ai-adenosine receptor, characterized by [3H] cy-clohexyladenosine binding, mediates the depression of evoked potentials in a rat hippocampal slice preparation. Neurosci Lett 28 275-9... 

An alternative mechanism for regulation of p42/p44 MAPK could be the release of the inhibitory regulation of c-Raf that results from the phosphorylation of Raf by PKA. CBi receptor/Gi-mediated inhibition of cyclic AMP production and reduction of PKA activity would promote a net dephosphorylation of c-Raf, thereby permitting the Raf kinase to serve as an activator of MEK in the p42/p44 MAPK activation module. Evidence for this pathway has been described for WIN55212-2-stimulated NlE-115 neuroblastoma cells (Davis et al. 2003) and hippocampal slice preparations (Derkinderen et al. 2003). 

An alternative approach to the pharmacological manipulation of receptors is to use knockout animals. CBi knockout mice have been produced, and high-frequency stimulation (100 Hz for 250 ms) induced significantly larger LTP of the CAl field EPSP in hippocampal slices prepared from CBu " mice compared to wild-type controls (Bohme etal. 2000). This was not associated with any detectable change in the amplitude of the half-maximal field EPSP evoked in the two groups. 

Cultured hippocampal slices prepared using the membrane insert method have been used to study process outgrowth (4-6), synaptogenesis (4,5), epileptogenesis (7), delayed neuronal loss (7—12), calcium homeostasis (12),... 

Fountain SB, Ting YL, Teyler TJ (1992) The in vitro hippocampal slice preparation as a screen for neurotoxicity. Toxicol In Vitro 6 77-87. 

Segal, M., 1980, The action of serotonin in the rat hippocampal slice preparation,/. Physiol. 303 423-439. 

Spencer, H. J., Gribkoff, V. K., Cotman, C. W., and Lynch, G. S., 1976, GDEE antagonism of iontophoretic amino acid excitations in the intact hippocampus and in the hippocampal slice preparation. Brain Res. 105 471-481. 

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