Hints for Column Chromatography of Proteins

Identify the unknown lipids by comparison with a set of known lipids (standards) of the same type, chromatographed in the same run on the same plate. [Pg.89]

A versatile detection reagent for lipids is iodine vapor. The dry plate is placed into a chamber containing one or two Petri dishes with crystals of solid iodine. Lipid-containing spots are colored yellow to brownish after few minutes. The color disappears quickly in contact with air. [Pg.89]

An additional general detection method is the oxidation by concentrated sulfuric acid. Spray the dry plate in a hood with concentrated sulfuric acid and then heat it in a drying oven to 130 °C. Organic substances yield dark brown spots. [Pg.89]

Lipids are visualized by spraying the plate with Soln. D and heating to 180 °C 15 min. [Pg.89]

For identification of phospholipids use Hanes reagent as described in Protocol 3.1.4. [Pg.89]

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