Hill plot Human

Fig. 2. Hill plot for oxygenation of human hemoglobin A as a function of the partial pressure (PO2) of molecular oxygen. The diagram at the right shows that the Hill coefficient will reach a limiting value of one at both extremes of ligand concentration. For this reason this cooperativity index is best measured at ligand concentrations near half-maximal saturation.

Figure 7.6 Oxygen association with myoglobin (Mb) and human hemoglobin (Hb). (a) The Hill plot (b) a plot of oxygen partial pressure against the degree of hemoglobin saturation with oxygen.

Figure 27-3 A, Oxygen dissociation curves for human blood with different plasma pH, but constant PCO2 of 40mmHg, a 2,3-diphosphoglycerol concentration in erythrocytes of 5.0mmol/L, and temperature at 37 C. B, A Hill plot. Conditions are the same as in A. DPG, 2,3 diphosphog ycerol.

A novel new technique employing fluorescence correlation spectroscopy has confirmed specific interactions between a tetramethyirhodamine-labeled kavain derivative and human cortical neurons. Human cortical neurons were incubated with 1 nM of the dye-labeled kavain derivative. A total binding of 0.55 nM was found after an incubation period of 60 minutes. Fifty percent of the total binding was specifically displaced in the presence of 1 XM non-labeled (+)-kavain. Evidence for these specific interactions was verified by a saturation experiment. Both the non-linear Scatchard plot and the n value of 1.58 0.07 in the sigmoid Hill plot indicated binding sites with different binding affinities (Boonen etal., 2000). 

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