Higher-Order Structure and HX-MS in the Biopharmaceutical Industry

Requires very little material High sequence coverage 

Relatively high sample throughput High experimental reproducibility 

Can detect a very small change in a large molecule 

At present, a few amino acids, however, single residue resolution with ETD/ECD (see Chapters 8 and 9) and/or multienzyme approaches (see Section 7.3 and Chapter 6) may be a reality soon [47, 48] 

An entire experiment can be performed with subnanomole levels of material 

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