High-throughput Measurement of Permeability Profiles

Human colon adenocarcinoma cell line used as an absorption model 

Madin-Darby canine kidney cell line 

Unstirred water layer (2.4 mm thickness in PAMPA, when plates are 

Moles of drug dissolved in the artificial membrane divided by total moles of drug (usually expressed as a percentage) 

Asymmetry ratio, defined as (Vd/VaXP /Pc0 )) where Vd and Va are donor and acceptor solution volumes, respectively, and the superscripts refer to the two possible directions of transport 

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Avdeef, A., High-throughput measurements of permeability profiles, in van de Waterbeemd, H. Lennemas, H. Artursson, P. (eds.), Drug Bioavailability. Estimation of Solubility, Permeability, Absorption and Bioavailability Wiley-VCH Weinheim, 2003 (in press)... 

Figure 9.7 Effective permeabilities for some weak acids, as measured by PAMPAs at three different pH values. pAa values for the acids are shown in parentheses. (Reproduced with permission from Avdeef, A., High-throughput Measurement of Permeability Profiles , Chapter 3 in Drug Bioavailability, van de Waterbeemd, H., Lennernas, H., Artursson, P., Eds. (Wiley-VCH Verlag GmbH Co. KGaA) p. 53, copyright 2003).

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