High-speed GPC

H., High-temperature coupling of high-speed GPC with continued viscome-try. II. Ethylene-vinyl acetate copolymers, /. Appl. Polym. Sci., 29, 1569, 1984. 

A systems approach to high-resolution, high-speed GPC takes all of these factors into consideration. Several aspects are worthy of detailed discussion. 

MILLER AND KERBER High-Performance High-Speed GPC... 

The recent introduction of high-speed GPC columns reduces time consumption even further and allows a complete 2D analysis in about an hour. 

Presently, the high-speed GPC procedures are rapidly gaining importance also in the field of separation of biologic materials. Typically, one needs 10-20 min for a complete HP GPC analysis, compared with 2-5 h necessary for conventional separations and even several times longer classical separations. Semiquantitative HP GPC separations lasting only about 1 -2 min were also reported using columns with very small volume and high flow rates. 

Evidently, at high-speed GPC separations, very severe requirements are laid also on the other parts of the apparatus, in the first instance on the pumping system, injector and volumeters. The constancy of all operational variables must be more rigorously controlled, as well. On the other hand, the application of smaller columns means material savings, especially as the amount of both sample and eluent is substantially reduced. 

Recently, high speed GPC has become used more and more frequently. It is carried out under increased pressure of the eluent and, therefore, special equipment has to be employed. Analytical uses thus prevail. The time required for analysis is several times shorter and more accurate results are obtained than with the conventional procedure. For details see section 4.6.3.9. 

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