High resolution separation column Latex

The porous glass packed columns did not yield high resolution separations, but the major species present in a latex were adequately separated. Figure 1. Insoluble polymer, when present, was excluded from the pores and eluted at interstitial volume. Elution order of remaining species was soluble polymer, unreacted monomers, and water. In both types of resins studied, no separation of the two unreacted monomers was achieved. A single chromatographic peak, that included both monomers, was obtained. 

A unique anion-exchange column has been developed that has a thin (non-diffusion limited) anion-exchange phase coated onto a 10-/nm latex bead. When a mobile phase of 0.15 M NaOH is used, neutral carbohydrates are converted into anions, which are separated on the column. Although the resin has low capacity, and probably causes degradation of the carbohydrates, when it is coupled to a triple-pulsed, amperometric detector, the system provides extremely sensitive, high-resolution separations. 

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