High resolution field desorption mass spectroscopy

Schulton W.D. Lehmman, High-Resolution Field Desorption Mass Spectroscopy. Part VII. Explosives and Explosive Mixtures , AnalChem-Acta 93,19-31 (1977) 153) A. Aim et al,... 

Obata and co-workers [7] separated stereoisomeric products formed during the synthesis of (2S, 3S)-2,3 bntane diyl (2S, 4S) 2, 4 pentane diyl and (2S, 5S) hexane diyl bis (4-vinyl benzoate) by chiral HPLC and characterised these polymers by high resolution field desorption mass spectroscopy (HR-FD-MS) and C-NMR. 

Field desorption mass spectrometry Heteronuclear multiple-quantum correlation Heteronuclear multiple-quantum correlation Homonuclear Hartmann-Hash spectroscopy High performance liquid chromatography High resolution electrospray ionization High resolution first atom bombardment massspectrometry... 

Further advancement in sequence determination by mass spectra was brought about through the introduction of new modes of ionization, such as field desorption and fast electron bombardment. Double focusing high resolution instruments and sophisticated computation greatly increased the number of peptides that could be sequenced by mass spectroscopy. For compounds which are unsuited for Edman degradation, such as cyclic peptides or peptides with an acyl group at their N-termini mass spectra can turn out to be the most practical avenue to sequence determination. 

Kassalainen and Williams [135] coupled thermal field flow fractionation (ThFFF) and matrix-assisted laser desorption/ionisation time-of-flight mass spectroscopy (MALDI-ToF-MS) to yield a powerful combination of techniques for the analysis of polydisperse PS. ThFFF high selectivity and sensitivity to chemical composition were used to separate polydisperse polymers and polymer mixtures into the narrow polydispersity and homogeneous chemical composition fractions essential for MAT.DT-ToF-MS analyses. On the other hand, because it is possible to measure directly using MALDI-ToF-MS, it alleviates the need for polymer standards for ThFFF. Kassalainen and Williams [135] address the coupling of ThFFF and MALDI-ToF-MS and identify compatibility issues. Optimum conditions were determined and developed to maximise the capabilities of the combined technique. Depending on the polymer and the method of matrix-assisted laser desorption/ionisation (MALDI) sample deposition, fractions from 1-10 ThFFF runs were combined for MALDI-ToF-MS analysis. Binary solvents are used to enhance ThFFF retention and resolution of low (<15 kDa) polymers, and methods developed to allow routine MALDI-ToF-MS analyses of PS polymers up to 575 kDa. Overall, the compatibility of the two techniques was extended from several kilodaltons to several hundred kDa. Polymer... 

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