High-pressure preparative chromatography detectors

Mahkam et al. 2009 prepared insulin-loaded modified alginate/chitosan matrix nanohydrogels. The modified nanohydrogels were characterized by SEM and Fourier transform infrared spectroscopy (FTIR). In vitro release studies were performed in acidic buffer (pH 1) and intestinal buffer (pH 7.4) with high-pressure liquid chromatography with ultraviolet detector at 210mn [43]. 

Column separation of proteins and peptides is used for both preparative and analytical purposes. Generally, in the former case low- or medium-pressure systems are preferred, whereas in the latter, high-performance-liquid-chromatography (HPLC) is the method of choice. These systems are coupled with a spectrophotometric detector normally set at wavelengths 280 and (around) 220 nm for proteins and peptides, respectively. Coupling of HPLC with mass spectrometry allows structural identification of compounds after the separation. 

A wide linear response range and high flow rate capability, are desirable features in a detector for preparative-scale chromatography. Problems with temperature stability (refractive index) and pressure pulses (UV detector) are less important because of the high sample concentrations present in the mobile phase. Some analytical detectors have interchangeable flow cells, which allow replacement of the analytical cell with one of... 

Figure 10 Enrichment and separation setup for SLM extraction. A - peristaitic pump, B - membrane device with hoiiow fiber instaiied into fused siiica capiiiary, C - iniet on the donor side, D - washing iine used after enrichment, E - microinjection pump to transport the acceptor into ioop F, G - Cis packed capillary column, H - detector window, I - splitter unit, J - high pressure pump. (Adapted from Thordarson E, Palmarsdottir S, Mathiasson L, and Jonsson JA (1996) Sample preparation using a miniaturized supported liquid membrane device connected on-line to packed capillary liquid chromatography. Analytical Chemistry 68 2559-2563.)...

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