High pressure liquid chromatography flow-cell

Brooker, G., Effect of temperature control on the stability and sensitivity of a high pressure liquid chromatography ultraviolet flow cell detector. Anal. Chem., 1971, 43, 1095... 

High performance liquid chromatography was performed with a high pressure liquid chromatography apparatus equipped with a U.V. detector (280 nm). The U.V. monitor has an 8 pi flow cell. The chromatographic column was a 20 cm x 2.1 mm (internal diameter) pre-packed stainless-steel Micro-Pack" column (Varian, Palo Alto, Calif.,... 

Fig. 12.20 Typej of amperomctric electrochemica] detector used in high-pressure liquid chromatography, (a) Tubular electrode, (b) Thin-layer channel cell, (c) Disc electrodes, (d) Rotating-disc electrode, (e) Wall jet. (f) Polarographic detector mercury Aow parallel to and in same direction as, electrolyte flow, (g) Mercury flow parallel to, and in opposite direction to, electrolyte flow, (h) Mercury flow perpendicular to electrolyte flow, (i) Porous electrode.

One variant, close to LC, that has been successfully interfaced to infrared spectroscopy, is supercritical fluid chromatography (SFC). In this case, one of the key mobile phases, super critical carbon dioxide, is virtually transparent for most of the mid-IR region. This may be operated directly on-line with a high-pressure liquid flow cell. 

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