High-performance size exclusion characterization

High-performance size exclusion chromatography is used for the characterization of copolymers, as well as for biopolymers (3). The packings for analyses of water-soluble polymers mainly consist of 5- to 10-/Am particles derived from deactivated silica or hydrophilic polymeric supports. For the investigation of organosoluble polymers, cross-linked polystyrene beads are still the column packing of choice. 

Pfannkoch, E., Lu, K. C., Regnier, F. E., and Barth, H. G., Characterization of some commercial high-performance size-exclusion chromatography columns for water-soluble polymers, /. Chromatogr. Sci., 18, 430, 1980. 

Pelekani, C., G. Newcombe, V. L. Snoeyink, C. Hepplewhite, S. Assemi, and R. Beckett. 1999. Characterization of natural organic matter using high performance size exclusion chromatography. Environmental Science and Technology 33 2807. 

Vachet, R.W. and M.B. Callaway. 2003. Characterization of Cu(II)-binding ligands from the Chesapeake Bay using high-performance size-exclusion chromatography and mass spectrometry. Matter. Chem. 82 31-45. 

The amylose fraction from three different oat starches was characterized by high-performance size-exclusion chromatography (HP-SEC) and shown to be free of amylopectin.31 The degree of polymerization is given in Table 15.2. By treating amylose with isoamylase, it was found to be branched.31 The major fraction had a DP of 700, whereas a second fraction had a DP of 72. The amylose chain length was reported to decrease with increased amylose and starch-lipid content.30 The intrinsic viscosity of amylose has been reported to be 246-299 mL/g.7... 

Johnson, D.K. Chum, H.L. "Some aspects of pyrolysis oils characterization by high performance size exclusion chromatography (HPSEC)." In This Volume. 

Some Aspects of Pyrolysis Oils Characterization by High-Performance Size Exclusion Chromatography... 

Carvalho et al. [108] studied chain degradation in TPS conqtosites of maize starch plasticized with glycerol and reinforced with wood pulp. The product was characterized by high performance size-exclusion chromatography (HPSEC). The matrix (starch/glycerol) and the composites were prepared in an intensive batch mixer at 150-160°C, with glycerol and fibre contents in the range of 30-50 per cent and 5-15 per cent, respectively. The HPSEC curves obtained for different levels of plasticization without fibre are shown in Fig. 15.5. 

A typical profile from a high-performance size exclusion chromatography (HPSEC) column is shown in Fig. 2. With ideal SEC systems, plots of the log of molecular weight against Kq produce calibration curves like those shown in Fig. 3. SEC supports of a single pore size with a narrow pore distribution should produce curves that are linear over a wide range of Kq values, usually 0.1-0.8. In order to achieve a linear calibration curve, it is essential that there be no Interaction between the column support material and the solute. Because solute-support interactions have been observed in every support material that has ever been introduced for SEC, the nature and characterization of these retentive phenomena will be discussed in detail later in the chapter. 

Jackson DS, Choto-Owen C, Waniska RD, Rooney LW. 1988. Characterization of steu ch cooked in tilkali by aqueous high performance size-exclusion chromatography. Cereal Chem 65 493-496. 

OR Larroque, MC Gianibelli, IL Batey, F MacRitchie. Electrophoretic characterization of fractions collected from gluten protein extracts subjected to size-exclusion high-performance liquid chromatography. Electrophoresis 18 1064-1067, 1997. 

Konas M, Moy TM, Rogers ME, Shultz AR Ward TC, McGrath JE (1995) Molecular weight characterization of soluble high performance polyimides. 1. polymer-solvent-stationary phase interactions in size exclusion chromatography, J. Polym Sci, Part A Polym Phys 33 1429... 

A HE CHARACTERIZATION OF MODERN HIGH-PERFORMANCE POLYMERS is still a challenge for polymer scientists. Copolymers and complex polymer blends play an important role in many applications (i). A fast, reliable, and comprehensive method is needed to succeed in this task. Size-exclusion chromatography (SEC) is a standard method for the determination of molar mass distributions (MMDs) and molecular weights, if... 

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