High-performance liquid chromatography plate height

High-Performance liquid Chromatography. Typical performances for various experimental conditions are given in Table 11.15. The data assume these reduced parameters h = 3, V = 4.5. The reduced plate height is... 

Figure 33-2 shows plots of plate heights // as a function of average linear velocity u in cm/s for high-performance liquid chromatography and supercritical-fluid chromatography. In both cases, the solute was pyrene, and the stationary phase was a reversed-phase octadecyl silane maintained at 40°C. The mobile phase for HPLC was acetonitrile and water, while for SFC the mobile phase was carbon dioxide. These conditions yielded about the same retention factor (k) for both mobile phases. Note that the minimum in plate height occurred at a flow rate of 0.13 cm/s... 

Particle size Plate height and column permeability decrease with the particle diameter. Smaller particle size columns provide, therefore, a better resolution. This concept, which led to the development of Ultra High Performance Liquid Chromatography (UHPLC), can also be adapted to SEC taking some peculiarities into account. Figure 9.10 shows a comparison of a protein mixture... 

The term plate height comes from distillation theory. Some high-performance distillation columns contain discrete units called plates, in which liquid and vapor equilibrate with each other. As a teenager, A. J. R Martin, coinventor of partition chromatography, built distillation columns in discrete sections from coffee cans. (We don t know what he was distilling ) When he formulated the theory of partition chromatography, he adopted terms from distillation theory. 

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