High-performance liquid chromatography method validation process

In most cases, the investigator should compare the results of the microchip analysis with those an offline standard technique (CE high-performance liquid chromatography, HPLC) for method validation. Control microdialysate samples must be collected, particularly for in vivo experiments that involve injection of a compound or drug. Such control samples can help identify or eliminate peaks from the microchip analysis. In many cases it is useful to incorporate an internal standard into the sampling process to correct for any changes in the recovery, reaction chemistry or with the on-chip injection process. 

Traditional Chinese medicines (TCMs) serve as a major source of a variety of drug lead compounds. In the process of natural products development, bioassay-guided isolation is a rapid and validated method for isolation of compounds with bioactivities. This chapter describes bioassay-guided separation and purification of compounds from the crude extracts of TCMs. Two approaches including size-exclusion chromatography (SEC) and high performance liquid chromatography (HPLC) are described in detail. 

---