High Affinity Complementation System

This technology is highly sensitive and DiscoveRx has developed several assays to measure cAMP [212], tyrosine or serine kinase [213], nuclear hormone receptors and proteases [214], as weU as assays for the measurement of cellular protein expression [211]. 

EDANS 5-((2 -aminoethyl)amino)naphthalene-l-sulfonic acid 

NADPH nicotinamide adenine dinucleotide phosphate hydride 

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Impaired function of phagocytes and deficiency of early components of the classic pathway of the complement system result in increased apoptotic waste. This waste, including nucleosomes and other autoantigens, is formed and altered by the protease of apoptotic cells. They are expressed on the surface of apoptotic blebs and activate bystander dendritic cells. Subsequently the dendritic cells activate helper T cells, which then help B cells to generate high-affinity autoantibodies (Fig. 1). 

Separation by partition between two immiscible aqueous phases has been successfully applied to a large number of proteins, nucleic acids, and cellular particles such as mitochondria, chloroplasts, membrane vesicles, and whole cells. The phase systems are made up of two different water-soluble polymers, dextran and poly(ethylene glycol), dissolved in water and complemented with suitable buffers, salts, and sucrose. Highly selective adjustment of the distribution process is possible by linking an affinity... 

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