Hexopyranosides

The mercuration-demercuration sequence was applied to the stereospecific synthesis of 2 deoxy-o-hexopyranoside derivative via a mercury(ll) trifluoroace-tate-promoted cyclization [54] (equation 25)... 

Methyl 2-deoxy-P-v- yxo-hexopyranoside. A suspension of 170 mg. of the nitrobenzoylated hexoside (obtained in the preceding preparation) in 50 ml. of 0.01M methanolic sodium methoxide is stirred for 4 hours at room temperature. The solvent is removed at 40°C. under diminished pressure, and the residue is suspended in 15 ml. of water. The suspension... 

By monitoring the intensity of the carbonyl absorption it was observed that oxidation of methyl 4,6-0-benzylidene-2-deoxy-a-D-Zt/ ro-hexopyrano-side with chromium trioxide-pyridine at room temperature gave initially the hexopyranosid-3-ulose (2) in low concentration, but attempts to increase this yield resulted in elimination of methanol to give compound 3. However, when methyl 4,6-0-benzylidene-2-deoxy-a-D-Zt/ ro-hexo-pyranoside is oxidized by ruthenium tetroxide in either carbon tetrachloride or methylene dichloride it affords compound 2 without concomitant elimination. When compound 2 was heated for 30 minutes in pyridine which was 0.1 M in either perchloric acid or hydrochloric acid it afforded compound 3, but in pyridine alone it was recoverable unchanged (2). Another example of this type of elimination, leading to the introduction of unsaturation into a glycopyranoid ring, was observed... 

Product 15 was eventually transformed into methyl 4,6-dideoxy-a-D-xylo-hexopyranoside by reductive desulfurization. A related reaction is the formation (41) of the 4-thiocyanato derivative 17 in 47% yield from 16 (140°C., 22 hours in N,N-dimethylformamide). This has been suggested as a good route to 4-deoxy-D-xt/Zo-hexoses compared with the available methods (43). 

In view of the unexpected effects of the C-2 and C-3 substituents on the reaction of C-4 sulfonates, it is worthwhile to point out the observations made with some 2,3-dideoxy derivatives. Treatment of ethyl 2,3-dideoxy-4,6-di-0-methylsulfonyl-D-ert/ hro-hexopyranoside (40) with sodium iodide and acetone at 115°C. caused the displacement of the C-6 mesylate group selectively to give 41. Catalytic hydrogenation then gave the corresponding ethyl 4-0-methylsulfonyl-2,3,6-trideoxy- -D-en/ /iro-hexoside in good overall yield (83%) (72). 

D-arab/no-Hexos-3-ulose Methyl p-o-xy/o-hexopyranosid-4-ulose... 

Methyl 3-deoxy-3-aza-a-D-nbo-hexopyranoside 2-Carb-34.2. Replacement by carbon... 

Two branched-chain sugars, methyl 3-azido-4,6-0-benzylidene-2,3,-dideoxy-3-C-(fluoromethyl)-a-D-flraZ)/ o-hexopyranoside and methyl 2-azido-4,6-0-benzylidene-2,3-dideoxy-2-C-(fluoromethyl)-) -D-r/to-hexo-pyranoside have been prepared through the usual displacement reactions. 

If k2 > kj, the glycosyl-enzyme intermediate will accumulate, and may be trapped by the rapid denaturation of the enzyme in the presence of (saturating) amounts of substrate. With -glucoside Aj from Asp. wentii and 4-nitrophenyl [ C]-2-deoxy-) -D-irra />jo-hexopyranoside, it was possible to identify the intermediate as a glycosyl ester (acylal) of 2-deoxy-D-arabino-hexose bound to the same aspartate residue that had previously been labeled with the active-site-directed inhibitor conduritol B epoxide and with D-glucal." This constituted an important proof that the carboxylate reacting with the epoxide is directly involved in catalysis. 

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